Abstract
We used Double Digest Restriction site associated DNA sequencing (ddRADseq), exome sequencing (exome-seq) and targeted genotyping by sequencing (GBS) to develop new geographically informative nuclear SNP markers in Picea abies. This set of 518 loci consists of 397 loci specifically designed for the geographic differentiation of populations and 121 loci of adaptive markers for drought stress which all were identified from 26 samples in 23 populations distributed over Central Europe. This set of novel markers represents a valuable basis to study the geographic population structure and genetic differentiation of Picea abies in its natural distribution range as well as outside of its native range with a focus on Central Europe.
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