A novel analytical, bioanalytical and theoretical approach to the platinum(II)-3-hydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one complex

Abstract

A concise, ingenious and expedite extractive spectrophotometric procedure for the micro-level analysis of Pt(II) using the novel synthesized Ligand of flavonoids family, 3-hydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one was elucidated by employing chelation as the basis of reaction between the two. The impetuous complexation and hence the chelation under basic conditions was revealed by the formation of dark brown coloured complex at an ambient pH of 8.79. The resulting brown coloured complex was extractable in non-aqueous organic solvents, the effectual and sensitive extraction and hence maximal colour intensity being exhibited in dichloromethane in the wavelength range 500–514 nm. The formed complex retained its adherence to the same colour intensity up to 5 days, thus validating its stability. The stoichiometric ratio as deduced by Job’s continuous variations, mole ratio and equilibrium shift methods of the formed Pt(II)-3-hydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one complex was ascertained to be 1:2 (M:L).The coherence to linearity was meticulously discerned in the concentration range 0.42–1.48 ppm of platinum(II). The corresponding ocular and statistical parameters of the resultant stable Pt(II)- 3-hydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one complex comprising of molar attenuation coefficient, Sandell’s sensitivity, %RSD, regression coefficient and limit of detection were evaluated as 7.1206×104 L/mol cm−1, 0.0027 µg cm−2,0.302 %, 0.9948 and 0.011 µg mL−1, respectively at 507 nm. To acquire a deeper knowledge of the complex's structural characteristics, theoretical investigations including Quantum theory of atoms in molecules and Natural bond orbital analysis were employed using Density functional theory calculations. The compound had been successfully used in therapeutic applications as an antibacterial, antioxidant and antineoplastic agent, in addition to its commercial analytical applicability.