Abstract
Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD+ concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 ± 0.67 µA/mM·cm2 and a detection limit of 20 µM. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy.
Highlights
Ethanol is one of the most important organic chemicals in human activities with a variety of uses in food industry, chemical synthesis, medicine and biotechnology
The surface morphology of Screen-printed electrodes (SPE), PAH/SPE and alcohol dehydrogenase (ADH)-sol-gel/PAH/SPE electrodes was studied by scanning electron microscopy (SEM)
The Scanning electron microscopy (SEM) micrograph of the PAH film deposited onto the SPE electrode (Figure 1B)
Summary
Ethanol is one of the most important organic chemicals in human activities with a variety of uses in food industry, chemical synthesis, medicine and biotechnology. Ethanol can appear as a bi-product in various processes in the food industry and can reach harmful levels due to fermentation and distillation. It causes alcohol poisoning in direct consumption by humans. For these reasons, ethanol detection is of great importance for clinical and industrial applications. Some of the most common detection methods for ethanol include high performance liquid chromatography [1], gas chromatography [2], capillary electrophoresis [3], colorimetry [4], Raman spectrometry [5], or tandem techniques [6,7]. Some of the above methods are complex and reliable, they can have disadvantages such as the need to use previous processes of sample separation (distillation, pervaporation), can be time consuming, and instruments can be expensive and need trained operators
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