A NOVEL AIRWAY STENT COATING TO REDUCE MUCOUS IMPACTION

Abstract

TOPIC: Biotechnology TYPE: Original Investigations PURPOSE: Collaborating with industry research partner Toray Industries, Inc., we developed and studied a hydrophilic coating for silicone stents in an effort to reduce mucous buildup. This coating would provide an opportunity for longer time to replacement for stents and ultimately better palliation of our patients' airway occlusion. Our goal is to study silicone stents with and without this coating in vitro via mucin adhesion test in order to quantify mucous buildup properties of both the coating and customized stents. METHODS: We modified commercially available silicone stent with each of three kinds of hydrophilic polymers according to Toray procedure. We used X-ray Photoelectron Spectroscopy (XPS) and Time-of-Flight Secondary Ion Mass Spectroscopy (TOF-SIMS) to provide surface analysis of the silicone stents. We conducted in vitro human mucin adhesion test comparing two silicone stents; one coated with the proprietary polymer (Stent-X) and the other without (Stent-C). Mucin adhesion was quantified with primary anti-human mucin antibody and secondary conjugate antibody. Our endpoint was to have 20% reduction in mucin adhesion with our coated stent vs non-coated stent. RESULTS: We successfully incorporated the proprietary coating to the surfaces of silicone stents and is visualized under TOF-SIMS (see Figure 1). Homogenous coating to commercially available stent was achieved. The advantageous properties of Stent-X include its high wettability coefficient and low friction coefficient. Bench testing using a mucin adhesion in vitro test demonstrated significant reduction of mucin at 24-hrs and at one-week incubation (see Figure 2). Stent-X produced an 80% reduction in mucin adhesion than Stent-C at one-week. CONCLUSIONS: Stent-X demonstrates promising anti-fouling capabilities in bench testing. Future work involving coated versus uncoated airway stents in a porcine model will help elucidate how the coating affects mucous buildup in an animal model airway stent. Subsequent design optimization is planned to incorporate both coating and individual anatomy in a silicone stent, with similar bench and porcine testing. CLINICAL IMPLICATIONS: The impact of an airway stent that offers anti-fouling properties would be significant with regard to reduced complications following stent implantation. DISCLOSURES: Consultant relationship with Toray Industries Please note: 1/2021 to 9/2021 Added 04/21/2021 by Roy Cho, source=Web Response, value=Grant/Research Support No relevant relationships by Daniel Glumac, source=Web Response No relevant relationships by Ryan Hunter, source=Web Response Employee relationship with Toray Industries Inc. Please note: From 2012 April Added 04/22/2021 by Koji Kadowaki, source=Web Response, value=Salary No relevant relationships by Leslie Kent, source=Web Response No relevant relationships by Robroy MacIver, source=Web Response No relevant relationships by Vidhu Pandey, source=Web Response No relevant relationships by Gregory Peterson, source=Admin input Employee relationship with Toray Industries, Inc. Please note: since 1991 Added 04/21/2021 by Kazuhiro Tanahashi, source=Web Response, value=Salary