Abstract
AbstractWe present a novel experimental platform based on a combined Atomic Force Microscopy (AFM) and Micro-Electrode Array (MEA) set-up. We have used it to measure minimal changes in the morphological/mechanical properties of electrically active cell cultures as well as to measure the changes in the extracellular electrical activity when a single cell is stimulated by means of the AFM tip. In particular, we studied the dynamical changes in cell elasticity of embryonic rat cardiac myocytes along the contraction-relaxation cycle. Applying high load indentations, we also recorded the effects of mechanical stimulations on the cell electrophysiology. The dynamic elastic modulus of the cell related to the contraction-relaxation cycle reveals a temporal behavior that closely follows the changes in cell height. Observed values of dynamic elastic modulus at a maximum indentation depth of 1500nm varied between 8.93 ± 0.78 kPa during systolic (contraction) phase and 4.26 ± 0.47 kPa during diastolic (relaxation) phase. Induced electrophysiological responses were observed when applying loads in the range 40-150 nN. The probability P of recording an induced electrical response (P = 0.16 for a maximum load of 100nN) increased with the maximum applied load. Pulling-like stimulations due to the tip-cell adhesion could also evocate electrical responses.
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