A novel acetogenic bacteria isolated from waste activated sludge and its potential application for enhancing anaerobic digestion performance

Abstract

The development of anaerobic digestion (AD) for volatile fatty acids (VFAs) production from waste activated sludge (WAS) is arrested due to low hydrolysis and acidification efficiency. This study proposed to enhance WAS reduction and VFAs accumulation during AD process via bioaugmentation of acetate-producing bacteria. Four acetogens were firstly isolated from a temperature-phased anaerobic digestion (TPAD) system. The acetate production efficiency of different isolates ranged from 15.8 to 73.7 mg acetate/g TOC, in which the bacterial strain NJUST19 was found to be the most effective strain. The results of morphological, biochemical characteristics as well as phylogenetic analysis showed that the isolate NJUST19 was Gram-positive and rod-shaped, catalase-negative, nitrate reduction-positive, methyl red-negative and capable of starch and gelatin hydrolysis, for which the name of Clostridium sp. NJUST19 was proposed. The optimal culture conditions (i.e. initial pH and temperature) were evaluated for their effects on microbe growth of selected NJUST19, and the maximum acetate production was observed at pH 9.0 and temperature of 40 °C. In the case of modified TPAD system inoculated with Clostridium sp. NJUST19, total suspended solids (TSS) removal rate and maximum VFAs accumulation increasing to 35.3% and 4200 mg/L, respectively, which was much higher than that of control (21.9% and 2894 mg/L). These results indicated that Clostridium sp. NJUST 19 is capable of enhancing digestion efficiency with a great benefit for VFAs production, offering potential prospects for bioaugmentation of WAS anaerobic digestion.