A Novel 3D-Printed Multi-Drive System for Synchronous Electrophysiological Recording in Multiple Brain Regions.

Jun Ma,Feng-Yu Liu,You Wan,Zifang Zhao,Shuang Cui,Ming Yi

doi:10.3389/fnins.2019.01322

Abstract

Extracellular electrophysiology has been widely applied in neural network studies. Local field potentials and single-unit activities can be recorded with high-density electrodes, which facilitate the decoding of neural codes. However, the chronic multi-regional recording is still a challenging task for achieving high placement accuracy and long-term stability. Here, we present a novel electrode design with low-cost 3D-printed parts and custom printed circuits boards. This new design could facilitate precise electrode placement in multiple brain regions simultaneously and reduce the working time for surgical procedures as well. In this paper, the design and fabrication of the 3D printed multi-channel microdrive are explained in detail. We also show the result of high-quality electrophysiological recordings in eight pain-related areas from rats and the electrode placement accuracy. This novel 3D-printed multi-drive system could achieve synchronous electrophysiological recording in multiple brain regions and facilitate future neural network research.

Highlights

To understand how our brain works, we have to observe the neural activities in different scales ranging from individual neurons to neuronal assemblies
A lightweight 3D printed multi-channel electrode with an independent drive system was developed for multi-site microelectrode implantation
The weight could be further reduced by adopting a thinner Printed circuit board (PCB) or replace the brass screw to titanium

Summary

Introduction

To understand how our brain works, we have to observe the neural activities in different scales ranging from individual neurons to neuronal assemblies. Different brain areas or nuclei form a functionally bound neural circuit and support various brain functions such as memory, fear, and pain (Buzsáki, 1989; Davis, 2003; Mizuseki et al, 2009; Bastuji et al, 2016) In this point of view, it is essential to record electrophysiological activities in multiple brain areas simultaneously. Longterm recording requires post-implantation adjustment of electrode localization, given the negative influences on signal quality from local inflammation from the surgery, vibration and neuronal degeneration (Williams et al, 1999). Another technical challenge is the electrode implantation.

Methods

Results

Discussion

Conclusion