A novel β-catenin from Apostichopus japonicus mediates Vibrio splendidus-induced inflammatory-like response

Abstract

β-catenin, an adaptor molecule in Wnt/β-catenin signaling pathway, is associated with different physiological processes such as intestinal immune, apoptosis, and inflammation-associated response. However, the function of β-catenin is still largely unknown in Apostichopus japonicus. In the present study, we cloned and characterized β-catenin gene from A. japonicus by RNA-seq and RACE approaches. The complete sequence of Ajβ-catenin consisted of a 5′ UTR of 166 bp, a 3′ UTR of 501 bp and an ORF of 2433 bp encoding a protein of 810 amino acids. Ajβ-catenin has a GSK-β consensus phosphorylation site of 21 amino acids located at N-terminal region and twelve Armadillo/β-catenin-like repeat (ARM) domains from 145 to 671 aa. Spatial expression analysis revealed that Ajβ-catenin mRNA levels displayed higher abundance in intestine. For Vibrio splendidus challenged sea cucumber, Ajβ-catenin transcripts reached their peak at 6 h and remained at higher levels until 24 h post infection in comparison with that of the control group. GSK-3β inhibitor treatment could induce both Ajβ-catenin and the inflammatory factors expression. Ajβ-catenin silencing could also down-regulate inflammatory factors expression. These results collectively suggested that Ajβ-catenin was a novel molecule mediate V. splendidus-induced immune response of A. japonicus via regulating the inflammatory factors expression.