Abstract
ABSTRACTThe two‐stage in vitro digestibility technique was scaled down to use 0.1 g samples. The accuracy of predicting in vivo DM digestibility by the use of micro‐samples was studied, using 35 samples of four grass species of known in vivo digestibility. When the sample size was reduced from the normal 0.5 g to 0.1 g, the residual standard deviation of the regression relating in vitro to in vivo DM digestibility was increased from ±2.5 to ±3.4 digestibility units. Grinding the samples more finely than through a 1.0 mm screen did not improve the accuracy of predicting in vivo digestibility.It was concluded that the in vitro method may be used with micro‐samples where necessary, but with less accuracy than the macro‐technique. For maximum accuracy, the use of standards of known in vivo digestibility is necessary.
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