Abstract
An attempt has been made to measure spectroscopically the Vitamin A content of pilchard oil. Using the same conversion factor as for cod liver oil, measurements of the extinction coefficient at 3280 Å gave Vitamin A values much larger than those obtained by biological tests. It has been shown that this discrepancy cannot be wholly ascribed to either the coloring matter or the saponifiable material in the oil. A satisfactory check on the results can be obtained only when it is possible to remove completely the Vitamin A from the oil, without otherwise modifying it.
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