Abstract
IN 1967 A feeding experiment was set up' involving 24 Holstein cows, allocated among 12 different feeding treatments, obtained as combinations of 4 hay-concentrate ratios and 3 feeding levels. The four ratios of concentrates to hay were chosen at the following percentage values of energy intake: 30/70; 45/55; 60/40; 75/25. Alfalfa hay and a suitable mix of concentrates were selected as feedstuffs.2 The three feeding levels were set at .32, .40, and .48 UF (energy measured in Scandinavian units of measurement) per kilogram of 4 percent fat converted milk, as projected from production of previous periods, plus the maintenance ration. It was recognized that, in principle, rations so specified are not in accord with the assumptions of classical regression analysis in which the treatment levels are chosen and fixed in advance by the experimenter. However, several considerations led to this approach in spite of the conceptual statistical disadvantage. First, because there were few available data concerning the lactation curves of cows under Italian feeding and environmental conditions, prespecification of relevant absolute rations would have been difficult. Second, the effects of the interdependence of observations between successive periods can be partially ameliorated by use of an autocor-
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