Abstract
The early diagnosis of prostate cancer is very vital for the improvement of patient survival chances. The content of prostate specific antigen (PSA) in serum is closely related to the status of the prostate cancer. We report a fluorescence bioassay, capable of detecting PSA in a non-enzymatic and label-free manner. PSA gives rise to the structural change of a hairpin, consequently triggering the hybridization chain reaction and forming a long-nicked double-helix, which is not adsorbed by graphene oxide. GelRed, as the signal indicator, then binds with dsDNA molecule, thereby producing the fluorescence. The established bioassay has the merits of simple operation, favorable cost-to-benefit ratios, good stability, and specificity. Moreover, the detection limit of this assay is as low as 10 pg/mL, and the linearity range is wide—from 100 pg/mL to 200 ng/mL. At the same time, this bioassay can realize the detection of PSA in biological samples (human serum, saliva, and urine). Therefore, the bioassay provides a potential means for the early diagnosis of prostate cancer.
Highlights
Prostate cancer (PCa) has become one of the most common tumors among men, especially in elderly males [1,2,3]
The methods utilized the labeled fluorescent probe. Inspired by these general studies, we present an enzyme-free fluorescence assay for the detection of Prostate specific antigen (PSA), by combination of Graphene oxide (GO) with hybridization chain reaction (HCR) and GelRed
PSA were added blankfor biological samples, human order to evaluate the of performance of this into bioassay the detection of including
Summary
Yujie Sun 1,† , Chenyun Wang 1,† , Hong Zhang 2 , Yulin Zhang 1, * and Guojun Zhang 1, *. Teaching and Research Office of Forensic Medicine, Hubei University of Chinese Medicine,. Received: 9 January 2019; Accepted: 19 February 2019; Published: 26 February 2019
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