Abstract
In this paper, a new principle biosensor for non-invasive monitoring of theregulation of photosynthetic metabolism based on quantitative measurement of delayedfluorescence (DF) is developed. The biosensor, which uses light-emitting diode lattice asexcitation light source and a compact Single Photon Counting Module to collect DF signal,is portable and can evaluate plant photosynthesis capacity in vivo. Compared with itsprimary version in our previous report, the biosensor can better control environmentalfactors. Moreover, the improved biosensor can automatically complete the measurements oflight and CO2 response curves of DF intensity. In the experimental study, the testing of theimproved biosensor has been made in soybean (Glycine max Zaoshu No. 18) seedlingstreated with NaHSO3 to induce changes in seedlings growth and photosynthetic metabolism.Contrast evaluations of seedlings photosynthesis were made from measurements of netphotosynthesis rate (Pn) based on consumption of CO2 in tested plants. Current testingresults have demonstrated that the improved biosensor can accurately determine theregulatory effects of NaHSO3 on photosynthetic metabolism. Therefore, the biosensorpresented here could be potential useful for real-time monitoring the regulatory effects ofplant growth regulators (PGRs) and other exogenous chemical factors on plant growth andphotosynthetic metabolism.
Highlights
Plant growth regulators (PGRs) and other exogenous chemical factors play important roles in regulating a wide range of physiological processes of plant growth and development such as photosynthetic metabolism, cellular differentiation, stomatal movements etc. [1]
A simple, non-invasive, and real-time method basing on light-induced delayed fluorescence (DF) technique for detecting changes of plant growth and photosynthetic metabolism caused by plant growth regulators (PGRs) and other exogenous chemical factors in seedlings is described in this study
Because DF is the most intrinsic sensitive fluorescence label of the photochemical efficiency of photosystem II (PSII) [8], the consistency of changes in DF and photosynthesis rate (Pn) suggested that the increase of Pn enhanced by low concentration NaHSO3 and the decrease of Pn inhibited by high concentration NaHSO3 could be attributed to the increase and decrease of photochemical efficiency of photosynthetic apparatus of mesophyll cells, respectively [3, 4]
Summary
Plant growth regulators (PGRs) and other exogenous chemical factors play important roles in regulating a wide range of physiological processes of plant growth and development such as photosynthetic metabolism, cellular differentiation, stomatal movements etc. [1]. Plant growth regulators (PGRs) and other exogenous chemical factors play important roles in regulating a wide range of physiological processes of plant growth and development such as photosynthetic metabolism, cellular differentiation, stomatal movements etc. Most of PGRs and other exogenous chemical factors always enhance photosynthetic metabolism at low concentrations but inhibit at high concentrations. In many areas of plant biology and agrochemical research, there is an increasing requirement for non-invasive and in vivo monitoring the effects of PGRs and other exogenous chemical factors on plant growth and photosynthetic metabolism [7]. A simple, non-invasive, and real-time method basing on light-induced delayed fluorescence (DF) technique for detecting changes of plant growth and photosynthetic metabolism caused by PGRs and other exogenous chemical factors in seedlings is described in this study. DF is a sensitive fluorescence label of the photochemical efficiency of charge separation at P680 and an excellent marker for evaluating in vivo plant photosynthesis ability with less interferences of environment [8]
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