Abstract
Porous anodic alumina (PAA) membranes have attracted considerable interest in biosensor research because they are simple to prepare and the size of the nanochannels can be controlled. However, traditional methods only utilize the porous layer as the barrier layer is removed before use. In this study, we propose a new biosensor for the detection of uric acid based on ion channels in the barrier layer of a PAA membrane. This exhibits a wide linear range (1 nM–0.1 mM) and has excellent selectivity in the presence of interferents (d‑(+)‑glucose, l‑ascorbic acid or dopamine). It is hoped that this new biosensor will prove useful in biological research and clinical medicine.
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