A non-classical crystallization mechanism of microbially-induced disordered dolomite

Abstract

Non-classical crystallization pathways, involving the formation of complex precursors prior to nucleation, have been observed during the calcification process of invertebrate skeletons and shells. However, these pathways were rarely documented in microbially-induced carbonate precipitation. In this study, we presented experimental evidence demonstrating that a halophilic bacterium (Halomonas sp. strain JBHLT-1) catalyzed the biomineralization of disordered dolomite through an amorphous calcium-magnesium carbonate (ACMC) with a stoichiometry near that of dolomite. During the ACMC formation, strain JBHLT-1 significantly enhanced the incorporation of Mg2+ ions compared to abiotic ACMC that was synthesized without any microbial biomass. In the stage of ACMC crystallization, the presence of microbial biomass reduced the dissolution-reprecipitation rate and induced bias towards a solid-state reaction pathway. This observation was supported by the negligible loss of structural Mg2+ ions in the presence of cells of strain JBHLT-1. The microbially-induced growth of disordered dolomite appeared to follow a spherulitic growth mechanism, as evidenced by its hierarchical structure composed of coalesced nano-spheres, eventually growing into micron-sized spheroids and dumbbells with extended incubation times. Since disordered dolomite is considered as a crucial crystalline precursor of ordered sedimentary dolomite, the findings of this study have important implications for understanding the formation mechanism of natural dolomite.