A noise delivery system for multi-animal multi-level whole body ototoxicity studies.

Abstract

The Naval Medical Research Unit Dayton (NAMRU-D) at Wright-Patterson Air Force Base, Ohio, in conjunction with the U.S. Air Force, studied ototoxic effects of JP-8 in rats. NAMRU-D used a multi-chamber whole body exposure facility for up to 96 test animals and 32 control animals at different exposure levels. The objective was to design a noise delivery system that could provide a white noise source one octave band wide, centered at 8 kHz frequency, delivered from outside the exposure chambers. Sound pressure levels were required to be within ±2 dB at all exposure points within each chamber and within ±2 dB over a 6-h run. Electrodynamic shakers were used to produce input noise in exposure chambers by inducing vibration in chamber plenums. Distribution of sound pressure levels across exposure points was controlled within a ±1.5dB prediction interval (α = 0.05) or better. Stability at a central reference point was controlled over 6-h runs within a ±1 dB prediction interval (α = 0.05) or better. The final system allowed NAMRU-D to deliver noise and whole-body aerosol exposures to multiple animals at different levels simultaneously and study the effects that ototoxins may have on hearing loss.