Abstract
The SCY1-like 1 binding protein 1 (SCYL1-BP1) protein was identified as an interacting partner of E3 ligase p53-induced RING H2 protein (Pirh2) and mouse double minute gene number 2 (MDM2) by yeast two-hybrid screening. Further investigation suggested there are two interactions involved in different mechanisms. SCYL1-BP1 can be ubiquitinated and degraded by Pirh2 but not by MDM2, which suggests that SCYL1-BP1 can be regulated by Pirh2. On the other hand, while SCYL1-BP1 binds to ubiquitin E3 ligase MDM2, it promotes MDM2 self-ubiquitination and results in a reduction of MDM2 protein level. Structured summary MINT- 7904819, MINT- 7904837, MINT- 7904806, MINT- 7904715: MDM2 (uniprotkb: Q00987) physically interacts (MI: 0915) with SCYL1-BP1 (uniprotkb: Q5T7V8) by anti tag coimmunoprecipitation (MI: 0007) MINT- 7904857, MINT- 7904899: SCYL1-BP1 (uniprotkb: Q5T7V8) physically interacts (MI: 0915) with MDM2 (uniprotkb: Q00987) by anti bait coimmunoprecipitation (MI: 0006)
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