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Abstract
Ephedra sinica polysaccharides have been reported to possess important activities, so quality evaluation of polysaccharides from the genus Ephedra is urgent. In this study, enzymatic digestions were performed to establish multiple saccharide fingerprints by ultra-performance liquid chromatography with electrospray ionization triple quadrupole linear ion trap mass spectrometry (UPLC-ESI-TQ-MS/MS) based on a multiple-reaction monitoring in negative mode. Under optimum UPLC-ESI--TQ-MS/MS conditions, excellent separation and quantification of 21 constituents were achieved within 20 min on a solid core column with a 1.6 μm particle using pre-column derivatization with a PMP reagent. This method, coupled with enzymatic digestions and principal component analysis, has been successfully applied to characterize and discriminate Ephedra polysaccharides attributed to different species and plant parts. The results suggest that the proposed analytical strategy could achieve a quality evaluation of plant polysaccharides from traditional Chinese medicines.
Highlights
Mahuang is a famous traditional Chinese medicine (TCM) that has been used for thousands of years for the treatment of allergies, asthma, pneumonia, bronchitis, hay fever, and colds [1].According to the 2015 edition of the Chinese pharmacopoeia, Mahuang is dry stems of Ephedra sinicaStapf, E. intermedia Schrenket C
ultra-performance liquid chromatography (UPLC)-electrospray ionization (ESI)− -TQ-MS/MS coupled with enzymatic digestions and principal component analysis (PCA) has been successfully applied to analyze and characterize 20 Ephedra polysaccharide samples attributed to different species and plant parts
The results showed that the proposed analytical methods provided reasonable insight and guidance for quality evaluation of plant polysaccharides from the genus Ephedra
Summary
Mahuang is a famous traditional Chinese medicine (TCM) that has been used for thousands of years for the treatment of allergies, asthma, pneumonia, bronchitis, hay fever, and colds [1]. According to the 2015 edition of the Chinese pharmacopoeia, Mahuang is dry stems of Ephedra sinica. It is essential to develop an effective method for quality evaluation of Ephedra polysaccharides. With the progress of chromatographic techniques, ultra-performance liquid chromatography (UPLC) was developed with a sub-2 μm diameter, which shortened the analysis time and provided high resolution and sensitivity separation, especially tandem mass spectrometry (MS/MS). UPLC-ESI− -TQ-MS/MS coupled with enzymatic digestions and principal component analysis (PCA) has been successfully applied to analyze and characterize 20 Ephedra polysaccharide samples attributed to different species and plant parts.
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