Abstract

SummaryFrozen and thawed cells of E. coli B and B/r lyse immediately upon addition of various T-bacteriophages (T2r+, T4D, T4Dr47, T3 and T7), at multiplicities of 1–15. Lysis is complete before the end of latent period and is more rapid as higher multiplicities of infection. When phage is added to thawed bacteria in 0.3 M sucrose, spheroplasts are formed.

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