Abstract

Our new type of circular dichroism (CD) spectrometer previously reported was successfully upgraded to have the capability of measuring CD spectra up to 200 nm and the total performance equivalent to commercial CD instruments. Optical components exchanged in the upgraded version are a 150 W Xe lamp, a crystal quartz Rochon prism, concave holographic gratings blazed at 250 nm, and a Hamamatsu R-292 photomultiplier with fused quartz window. It is revealed that a depolarizer should be mounted on a rotatable stage equipped with a mechanism for precise adjustment and be rotated carefully to find its best azimuth angle that makes the CD baseline shift as small as possible over the entire range of wavelength.

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