Abstract
Abstract This paper describes a new turbidometric assay for quantifying antibody-independent serum killing via the classical complement pathway. The assay was developed to assess the effect of ascorbate nutriture on complement-mediated host defense in humans. It gives results comparable to those obtained by the conventional colony counting method, but it is simpler, faster and gives better precision. The assay uses Salmonella minnesota R 595, a bacterium known to be particularly sensitive to the antibody-independent bactericidal activity of serum. At the serum concentrations used in this assay (less than 1.0%), the method is specific for classical pathway activity. The entire assay, including the final quantification of turbidity, is carried out in a single disposable tube. Although the inoculum requires overnight growth, the assay itself can be completed in a single working day. The assay requires less than 100 μL sterile serum. When data are plotted logarithmically, bacterial survival after exposure to ...
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