A NEW TECHNIQUE FOR HIGH-RESOLUTION DENSITY GRADIENT-SEPARATION OF BONE-MARROW CELLS.

Abstract

A new technique with minimal artifacts was developed for high-resolution density-gradient separation of viable cells. Differences as small as 0.002 in the density of the cells can be easily detected. It has been possible to detect differences of 0.0005 with careful manipulation. The development of this technique has necessitated the construction of a new centrifuge head, the preparation of a new suspension medium, and a new density gradient, and finally the development of a new centrifuge tube. Results on experiments on the bone marrow of three dogs are reported.