Abstract
A simple procedure, called the “cold acetone (-70°C) -dropping affixation technique”, for affixing fresh, frozen sections to cold, clean, untreated glass slides is described. A few drops of absolute acetone (-70°C) were dropped with a precooled pipet to the sections placed on the slides, and the acetone on the slides was absorbed immediately with a cold filter paper. The slides with the sections were left in a cryostat for approximately 30 min, so that the sections were affixed firmly to the slides. By this method we could prevent the diffusion of low-Km aldehyde dehydrogenase (ALDH) from the sections.The localization of low-Km ALDH activity in the hepatic lobule of normal mice was studied histochemically with the nitroblue tetrazolium (NBT) method. Reaction products, purplish-blue formazan showing the enzymatic activity, were found mainly in the centrilobular zone. Cyanamide (5 mM) inhibited the low-Km ALDH activity, whereas the high-Km ALDH activity was unaffected even by 10 mM cyanamide.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
