Abstract
A useful vector, pAGE103, has been developed for the expression of cDNA in animal cells using the simian virus 40 (SV40) expression signals. cDNA could be expressed easily by inserting it into the multiple cloning sites (HindIII, SalI/AccI, XbaI, BamHI, SmaI/XmaI, KpnI/Asp718, SacI and EcoRI) of the vector, which are located between the SV40 early promoter and the SV40 early RNA processing signals for splicing and polyadenylation. In addition to the above transcription unit, pAGE103 contains the replication origin of ColE1, and a dual KmR/G418R selective gene. Several unique restriction sites are located on the boundaries between the above-mentioned three components of the vector, allowing the easy substitution or insertion of other genetic elements. The human interferon-beta gene was inserted into pAGE103 and shown to be expressed transiently in COS-1 cells and stably in several animal cell lines.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.