A new surface plasmon resonance system with the function transforming freely from wavelength to angle modulation and the primary application in real time detection of antibody-antigen interaction

Abstract

Surface plasmon resonance (SPR) sensor has been widely investigated as an exceedingly powerful tool for biological and chemical detection in the past decay. As an essential component of both academic and commercial laboratories, several methods have been employed to monitor the excitation of SPR by measuring the light reflected from the sensor interface including modulation of angle, wavelength, intensity, phase and polarization. Among these methods, angle modulation (AM) and wavelength modulation (WM) are the most widely adopted in practice. However, the normal sensors system of the angle modulation was built by two ratable stages with a flow cell attaching a prism, and the angle of incidence rottingθ, the angle of reflect rotting 2θ. The mechanical structure can only be used in the angle modulation SPR sensor. In the present study, a new SPR is designed by a mechanical structure which can transform freely from wavelength to angle modulation. The different concentration Ethanol solution is tested by this system, and the linear range is very well from the volume ratio 0% to 60%. The primary application is done in real time detection of human IgG and antibody interaction, and the experimental results indicate that the new SPR system can detect human antibody at low concentration 1x10^-11g/ml with highly sensitivity and flexibility. The new SPR system is of great importance for potentially practical application in biosensor and life science with the extended the functions of the conventional SPR sensor, and provides the real-time and in-situ detection of the biomolecules.