Abstract
The detection of proteins is of great biological significance as disease biomarkers in early diagnosis, prognosis tracking and therapeutic evaluation. Thus, we developed a simple, sensitive and universal protein-sensing platform based on peptide and graphene oxide (GO). The design consists of a fluorophore (TAMRA, TAM), a peptide containing eight arginines and peptide ligand that could recognize the target protein, and GO used as a quencher. To demonstrate the feasible use of the sensor for target detection, Bcl-xL was evaluated as the model target. The sensor was proved to be sensitive and applied for the detection of the target proteins in buffer, 2% serum and living cells.
Highlights
Proteins play key roles in a wide range of human physiological processes, including catalysis, signaling, transport and storage, nutrition, immunity and regulation
A highly sensitive and selective fluorescent turn-on assay for signaling target protein was developed by utilizing peptide-graphene oxide (GO) sensors
This peptide-GO sensor design consists of a protein was developed by utilizing peptide-GO sensors
Summary
Proteins play key roles in a wide range of human physiological processes, including catalysis, signaling, transport and storage, nutrition, immunity and regulation. The expression of many proteins varies significantly between healthy people and patients with various diseases, which results in pathological changes [1,2]. Numerous methods have been reported to detect target proteins, such as enzyme-linked immunosorbent assay (ELISA), electrochemical immunology sensors and mass spectra analysis [7,8,9,10,11,12]. These methods have some limitations; for example, most involve multistep and time-consuming procedures and often cannot be well applied to intracellular detection. New strategies for rapid, convenient and sensitive protein detection are needed
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