Abstract
AbstractAlpha‐amylase is one of the most important enzymes in the saliva. It is an important biomarker for many health conditions. Saliva sampling is a non‐invasive method and is the preferred choice for sampling at point of the care and home testing. Measurement of salivary alpha‐amylase activity as a rapid test using test strips with Flavin‐dependent glucose dehydrogenases (FAD‐GDH) as glucose is the aim of the current research. FAD‐GDH is an oxygen‐independent enzyme and has a narrow substrate specificity. For this goal, starches from various sources are tested as a substrate for alpha‐amylase and chose MS‐101 starch as the best substrate, between six different starch types. Under optimal assay conditions, when hydrolyzed starch (in 20 min, 37 °C, pH = 7) reacts with FAD‐GDH enzymes that exist in test strips for 5 s glucometer can show the amount of glucose produced in samples from patient samples. This glucose value is directly correlated with the salivary alpha‐amylase activity (p‐value <0.01).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
