A New Spiropyran-Based Fluorescent Probe for Dual Sensing of Ferrous Ion and pH.

Abstract

A new spiropyran-based fluorescent probe was developed for dual detection of Fe2+ ion and pH. Addition of Fe2+ and Ag+ to the probe solution enhanced the fluorescence intensity by 6 and 5 fold, respectively. Addition of Fe3+, Hg2+ and Ni2+ caused slight increase in the fluorescence intensity of the probe. While addition of other common metal ions did not bring about substantial change of the fluorescence. Thus the probe can be used for fluorescence turn-on detection of Fe2+ ion in ethanol/water (9:1) medium. The detection limit of the probe for Fe2+ is 0.77 µM. The suitable pH range for the probe to detect Fe2+ was pH 3 - 9. Other metal ions including Li+, Na+, K+, Ag+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Sr2+, Hg2+, Ca2+, Mg2+, Al3+, Cr3+, and Fe3+ did not cause marked interference with Fe2+ recognition. The color of the probe solution was yellow at pH 1 - 2 and colorless at other pH values. The fluorescence intensity of the probe was low at pH 1 - 12 and increased significantly when the pH was 13 and 14, indicating that the probe can be used as a colorimetric and fluorescent probe for sensing extremely acidic or extremely alkaline conditions through different channels.