A new species of the genus Heterobothrium (Monogenea: Diclidophoridae) parasitizing the gills of tiger puffer fish Tetraodon lineatus (Tetraodontidae). A light and scanning electron microscopic study

Abstract

In the present study, the morphology and morphometric characterization of Heterobothrium lineatus, a monogenean gill parasite infecting the gills and wall of the bronchial cavity of the tiger pufferfish Tetraodon lineatus, were described by means of light and scanning electron microscopy for the first time from the River Nile at Qena Governorate, South Valley, Egypt. In wet mount preparation, the adult worms exhibited an elongated body with anterior pointed and posterior broad ends. The adult worm measured 1.15-1.76mm (1.53 ± 0.2) in length and 0.30-0.42mm (0.35 ± 0.02) in width. Light and scanning electron microscopic observations showed the presence of two buccal organs situated anteriorly around the mouth opening. The opisthohaptor was subdivided into four pairs of clamps but had no isthmus separating it from the body proper. The present Heterobothrium species differs from all other described species in the genus, by its lower dimensions of the worm measurements and the presence of a copulatory organ armed with 12-15 genital hooks. Furthermore, it is easily distinguished from Heterobothrium tetrodonis and Heterobothrium okamotoi by the absence of a distinct isthmus, and resembles Heterobothrium lamothei described from the gills of Sphoeroides testodineus in Mexico in its general appearance and the presence of rectangular haptor with the fourth pair of clamps smaller than the previous ones.