A new set of microsatellite loci for Leptonycteris yerbabuenae and cross species amplification with other glossophagines

Abstract

A technique based on 454 sequencing of an enriched library was used to construct genomic libraries highly enriched for microsatellite loci for Leptonycteris yerbabuenae. Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. We probed ascertainment bias in 5 different glossophagines (L. nivalis, Glossophaga leachii, G. soricina, Anoura geoffroyi, and Choeronycteris mexicana), indicating their potential utility for suitable studies of population genetics and other related analyses. Levels of expected heterozygosity were medium–low for all markers (mean HE = 0.147, range 0.008–0.46).