Abstract
Abstract A new sequential Optimization Procedure by Search Point (OPSP) based on Hooke-Jeeves algorithm is developed. The procedure is an automated multifactor optimization of conditions for an HPLC separation. Its usefulness in computer-assisted method development is shown by the experimental mobile phase optimization of an isocratic reverse phase liquid chromatography separation of a mixture of six selected pesticides. The relative composition of a ternary mobile phase (Acetonitrile, Methanol, Water) was varied during the optimization process. An objective function (OF) which was used as the criterion of quality of the chromatographic separation is described. The perfomance of this new chromatographic method is evaluated either by plotting the map of the separation quality using a Grid Search method or by comparing the results with the ones obtained by the application of a Modified Simplex method, in both cases over the same triangular (Acetonitrile, Methanol, Water) parameter space. The optimum solvent...
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