Abstract
A new method has been developed for the determination of serum carnosinase activity using l-carnosine-[1- 14C]β-alanyl as substrate. The serum carnosinase activity was calculated from the ratio decomp./min β-alanine: total decomp./min at the end of the incubation. The K m -value of the serum carnosinase-catalysed hydrolysis of l-carnosine was found to be 7.1 · 10 −5 M. In adults the mean value for serum carnosinase activity amounted to 44.2 ± 14 (S.D.) units/1. In serum of newborns only very low carnosinase activities were found. This sensitive method may be applied to detect patients with serum carnosinase deficiency.
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