Abstract
A new simple precise, accurate and selective RP-HPLC method was developed for the simultaneous estimation of Paracetamol and Aceclofenac in their mixed formulation by separating Diclofenac. All the three drugs were separated on Kromasil C18 (150x4.6, 5μ) with reverse phase elution of the mobile phase compose of 0.05M potassium dihydrogen phosphate and Acetonitrile in the ratio 40:60 v/v at a flow rate of 1.0mL/min. The detection was made at 275nm. The retention times were 2.36 min for Paracetamol, 3.23 min for Aceclofeanc and 6.38 min for Diclofenac. The linearity ranges for paracetamol and aceclofenac were 16.25 to 48.75 and 5 to 15 mcg/ml respectively with correlation coefficients 0.999 and 1.0. The proposed method statistically validated with respect to system suitability, specificity, linearity, range, precision, accuracy, robustness and ruggedness. The method was accurate, linear, precise, specific, selective and rapid found to be suitable for the quantitative estimation of Paracetamol and Aceclofenac by separating Diclofenac.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
