Abstract
A simple, sensitive and selective fluorescent chemo dosimeter rhodamine B phenyl hydrazide (RBPH) for Cu2+ was proposed. This probe is non fluorescent and colorless but exhibits fluorescent enhancement at 580nm and displayed color change from colorless to pink for Cu2+ in the pH range 1–6. Fluorescence microscope experimental results reveals that this chemo sensor is cell permeable and can be used for fluorescence imaging of Cu2+ ions in living cells. This probe can detect Cu2+ with good linear relationships from 10 to 100nM with r=0.99971 then limit of detection was found to be 0.015nM with ±0.91% RSD at 10nM concentrations.
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