Abstract
By using the cDNA probe for the human alpha-fetoprotein (AFP) gene, one restriction fragment length polymorphism (RFLP) was discovered in Japanese patients. A variety of combinations of restriction enzymes and probes were evaluated in DNA samples from 25 Japanese individuals who were genetically unrelated. As a result, AvaII identified one invariant band of 8 kb and polymorphic bands at either 2.8 or 2.0 kb with the 5'-region probe from a cDNA clone, pHAF7. With the AvaII/pHAF7 combination, we examined the loss of heterozygosity in 14 patients with hepatocellular carcinoma (HCC). Of 6 informative specimens, 2 showed loss of heterozygosity. These data suggest that the AFP cDNA probe could be useful for analyzing loss of heterozygosity on chromosome 4q in human HCC.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
