Abstract
The officially accepted Protein Efficiency Ratio (PER) assay has limited applicability as a quality control and process monitoring assay due to the 28 days required to complete the assay. The ultimate measure of protein nutritional quality is the human bio-assay; however, this takes from 35–45 days to complete and is very expensive compared to the PER assay. PER is recognised by many nutritional biochemists as only an interim measure of human protein quality. The need for a reliable, timely, inexpensive rapid protein quality assay has become more evident in recent years with many rapid methods, including the Pepsin Digest Residue (PDR), Pepsin Pancreatin Digest (PPD), Pepsin Pancreatin Digest Dialysate (PPDD) and the Calculated Protein Efficiency Ratio (C-PER) methods, appearing in the research literature over the past 20 years. While each assay has notable merits, inherent limitations associated with each prevent use on an industrial basis. We now report a rapid assay, the Pepsin Digest Dialysate (PDD) Index, that can be used to determine protein quality over a wide range of protein sources currently available in human foods and which is acceptable for use in the food and nutritional product industries and for the Food and Drug Administration.
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