Abstract
A new method for the determination of adenosine 5′-phosphosulphate (APS) kinase activity using a spectrophotometric assay is described. This procedure involves the spectrophotometric determination of sulphate- or APS-dependent production of ADP in the presence of pyruvate kinase and lactate dehydrogenase. Methods are described that overcome interference from contaminating enzymes and compounds. This method also provides a means for a critical examination of the substrate specificity of the sulphate-activating enzymes.
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