A new radioimmunoassay of free thyroxine using 125I-labelled thyroxine—protein complex uninfluenced by albumin and thyroxine-binding globulin

Abstract

We describe a double-antibody solid phase radioimmunoassay for free thyroxine (FT 4) in serum with use of 125I-labelled thyroxine-human chorionic gonadotropin conjugate. Since the labelled conjugate does not bind to thyroxine binding globulin (TBG) and albumin because of its large molecular weight, the method is uninfluenced by TBG or albumin. The measurable range of FT 4 in serum was 2.0 to 128 ng/l. The mean coefficients of variation within and between assays were 4.6–8.6% and 6.3–11.6%, respectively. The FT 4 values determined by the proposed method correlated well with those determined by commercial radioimmunoassay in subjects with normal albumin concentration ( r = 0.98). The FT 4 concentrations in serum as determined by this method were 9 to 17 ng/l for healthy adult subjects; high for patients with hyperthyroidism; low for patients with hypothyroidism; and within normal limits for pregnant women, and patients with high or low concentrations of thyroxine-binding globulin.