A New Procedure for the Multiplex PCR Amplification of TH01, CSF1PO and TPOX Loci with Small Amplicon Lengths.

Abstract

We describe a new procedure for the multiplex PCR amplification of the three short tandem repeat (STR) loci; TH01, CSF1PO and TPOX with small amplicon lengths (the Small TCT Multiplex). The accuracy of the Small TCT Multiplex was verified by typing 100 Japanese samples that had been previously typed using an AmpFlSTR Green I PCR amplification kit (Green I Kit). The results using the Small TCT Multiplex were consistent with those obtained by the Green I Kit. STR typing using the Small TCT Multiplex was examined from 36 bloodstain samples that had been left for 1 to 25 years at room temperature and compared to that using the Green I Kit. The Small TCT Multiplex was superior to the Green I Kit for STR typing especially in the case of bloodstain samples that had aged for more than 8 years.