Abstract
We report a new type of potentiometric immunosensor for the determination of human α-fetoprotein (AFP). Gelatin–silver film as gentle carrier was used to immobilize anti-AFP on the surface of platinum disk electrode. The effective immobilization of the antibody comes from the entrapment by the gelatin film and graft by silver nanoparticles. Glutaraldehyde was employed to improve the character of complex film. Cyclic volatmmograms (CV) and electrochemical impedance spectroscopy (EIS) were used to determine the character of the film constituting the active phase of the electrode. The resulting AFP sensor exhibited a short response time (<5 min), high sensitivity, low interference from other antigens, and a working lifetime at least 60 days. The analytical results by the proposed method were in satisfactory agreement with those by enzyme-linked immunosorbent assay (ELISA). It would be valuable for clinical immunoassay.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
