Abstract
A new phenothiazine‐based sensor PHE‐Ad for monitoring Hg2+ has been designed and synthesized based on the intramolecular charge transfer (ICT) mechanism. The probes were characterized by FTIR, 1H NMR, and HRMS, and their optical properties were detected by UV and FL. It's showed the probes detection of Hg2+ compared to other metal ions (Mg2+, Cu2+, Hg2+, Ag+, Co2+, Cr3+, Al3+, Ni2+, Zn2+, Ca2+, Fe3+, Fe2+, K+, Na+, and Cd2+) based on the test results. Besides, the detection limits were determined to be 2.12 × 10−8 M through the standard curve plot. In addition, sensor PHE‐Ad shows high selectivity and sensitivity for Hg2+ with a fast response in a suitable pH range. Furthermore, taking into account its good “turn‐on” fluorescent sensing behavior and low cell cytotoxicity, PHE‐Ad was successfully applied to detect and image Hg2+ in real water samples and living cells, which shows great potentials for application in environmental and biological systems.
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