A New Phase for WNK Kinase Signaling Complexes as Biomolecular Condensates.

Abstract

The purpose of this review is to highlight transformative advances that have been made in the field of biomolecular condensates with special emphasis on condensate material properties, physiology, and kinases, using the With-No-Lysine (WNK) Kinases as a prototypical example. To convey how WNK kinases illustrate important concepts for biomolecular condensates, we start with a brief history, focus on defining features of biomolecular condensates, and delve into some examples of how condensates are implicated in cellular physiology (and pathophysiology). We then highlight how WNK kinases, through the action of "WNK droplets" that ubiquitously regulate intracellular volume, and kidney-specific "WNK bodies" that are implicated in distal tubule salt reabsorption and potassium homeostasis, exemplify many of the defining features of condensates. Lastly, this review will address the controversies within this emerging field and questions to address.