Year-end Sale % OFF 
Abstract
Differentiation of the fish-borne trematodes belonging to the Opisthorchiidae, Heterophyidae and Lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the eggs are morphologically similar. Epidemiological investigation therefore currently relies on morphological examination of adult worms following expulsion chemotherapy. A PCR test capable of amplifying a segment of the internal transcribed spacer region of ribosomal DNA for the opisthorchiid and heterophyid flukes eggs taken directly from faeces was developed and evaluated in a rural community in central Thailand. The lowest quantity of DNA that could be amplified from individual adults of Opisthorchis viverrini, Clonorchis sinensis and Haplorchis taichui was estimated at 0.6 pg, 0.8 pg and 3 pg, respectively. The PCR was capable of detecting mixed infection with the aforementioned species of flukes under experimental conditions. A total of 11.6% of individuals in rural communities in Sanamchaikaet district, central Thailand, were positive for ‘Opisthorchis-like’ eggs in their faeces using conventional parasitological detection techniques. In comparison to microscopy, the PCR yielded a sensitivity and specificity of 71.0% and 76.7%, respectively. Analysis of the microscopy-positive PCR products revealed 64% and 23% of individuals to be infected with O. viverrini and C. sinensis, respectively. The remaining 13% (three individuals) were identified as eggs of Didymozoidae, presumably being passed mechanically in the faeces following the ingestion of infected fishes. An immediate finding of this study is the identification and first report of a C. sinensis–endemic community in central Thailand. This extends the known range of this liver fluke in Southeast Asia. The PCR developed herein provides an important tool for the specific identification of liver and intestinal fluke species for future epidemiological surveys.
Highlights
It is estimated that approximately 17 million people are currently infected with fish-borne trematodes worldwide [1]
Previous parasite surveys have mostly focussed on these communities and frequently found infection with O. viverrini mixed with minute intestinal flukes of the Heterophyidae and Lecithodendriidae [5,6]
The minute intestinal flukes are widely distributed in southeast Asia and are increasingly recognised as an emerging pathogen associated with diarrhoea and gastritis
Summary
It is estimated that approximately 17 million people are currently infected with fish-borne trematodes worldwide [1]. Previous parasite surveys have mostly focussed on these communities and frequently found infection with O. viverrini mixed with minute intestinal flukes of the Heterophyidae and Lecithodendriidae [5,6]. Microscopic examination of faecal samples for the presence of eggs using the formalin-ether concentration technique (FECT) is currently considered the most sensitive and reliable method for screening liver and intestinal flukes and is the most widely employed technique for fluke parasite surveys [7]. This technique is limited by its capacity to differentiate between the Opisthorchiidae, Heterophyidae and Lecithodendriidae, which have similar egg morphologies. C. sinensis has a wider definitive host range than O. viverrini [11,12] which makes control more challenging
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
