Abstract
This paper describes the development and performance of the first fructose biosensor based on a commercial screen-printed graphene electrode (SPGE). The electrode was modified with an osmium-polymer, which allowed the efficient wiring of the enzyme fructose dehydrogenase (FDH). The immobilization of both osmium-polymer and FDH was realized in an easy way. Aliquots of 10μL Os-polymer and 10μL FDH were thoroughly mixed with poly(ethylene glycol) (400) diglycidyl ether (PEDGE) and deposited on the electrode surface and left there to dry overnight. The biosensor exhibits a detection limit of 0.8μM, a linear range between 0.1 and 8mM, high sensitivity to fructose (2.15μAcm−2/mM), good reproducibility (RSD=1.9%), fast response time (3s) and a stability of 2 months when stored in the freezer.The proposed fructose biosensor was tested in real food samples and validated with a commercial spectrophotometric enzymatic kit. No significant interference was observed with the proposed biosensor.
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