Abstract
A new N-methoxypyridone analog (1), together with four known compounds, was isolated from the co-culture of Hawaiian endophytic fungi Camporesia sambuci FT1061 and Epicoccum sorghinum FT1062. The structure of the new compound was elucidated as 11S-hydroxy-1-methoxyfusaricide (1) by extensive spectroscopic analysis and comparison with the literature. The absolute configuration of 1 was determined by comparison with the experimental and calculated ECD spectra. The absolute configuration of compound 3 was investigated and renamed as (+)-epipyridone by comparison of the optical rotation and CD spectrum with those of 1. The other known compounds were identified as epicoccarine B (2), D8646-2-6 (4), and iso-D8646-2-6 (5). Compounds 4 and 5 showed modest inhibitory activity towards pathogenic fungi. Epicoccarine B (2) inhibited A2780 and TK-10 with an IC50 value of 22 μM.
Highlights
Manipulation of fermentation conditions of fungi has been proven to be an efficient strategy for obtaining diversified compounds [1,2,3]
The fungal strains Camporesia sambuci FT1061 and Epicoccum sorghinum FT1062 were isolated on PDA medium from a healthy fruit of the plant Rhodomyrtus tomentosa collected on the Big Island in Hawaii in 2016
Microbial communication can lead to the activation of silent fungal secondary metabolite gene clusters [36], which has been proved to be a potentail way to enhance chemical divesity [37]
Summary
Manipulation of fermentation conditions of fungi has been proven to be an efficient strategy for obtaining diversified compounds [1,2,3]. Co-cultivation of two or more strains has recently been described as a promising strategy for inducing the production of bioactive microbial metabolites [4,5]. In addition to obtaining new metabolites [6,7,8,9], the strategy can be used to increase the yields of previously described bioactive compounds [10]. During our continuing investigation of Hawaiian endophytic fungi [15,16,17,18,19,20,21,22], two endophytic fungal strains, FT1061 (Camporesia sambuci) and FT1062 (Epicoccum sorghinum) were isolated from Rhodomyrtus tomentosa (downy rose myrtle) [23], an invasive pest plant in the State of Hawaii.
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