Abstract
Objective The aim of this study was to examine a new method to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome. Methods A senna leaf (Folium sennae) decoction was made in 3 concentrations of 1.0, 0.5, and 0.25 g/mL. Rats were randomly divided into 4 groups: the control (C)-, high (H)-, middle (M)-, and low (L)- dose groups. The groups received saline, 1.0, 0.5, or 0.25 g/mL senna leaf decoction, respectively, for 4 weeks. Body weight monitoring, food consumption, water intake, defecation frequency, stool Bristol score, weight-loaded forced swimming test, forelimb grip strength test, D-xylose absorption test, serum cortisone, adrenocorticotropic hormone (ACTH), 24 h urine 17-hydroxycorticosteroid (17-OHCS), and histopathological detection were conducted to assess the success of the senna leaf decoction-induced model. Results This study showed that the senna leaf decoction could induce diarrhea and dose-dependently slow body weight growth, reduce food consumption, and increase water intake, stool Bristol score, and defecation frequency. Statistical differences were found between groups H and M in rectal temperature, weight-loaded forced swimming time, forelimb grip strength, and serum cortisone. The D-xylose absorption test also showed dysfunction of intestinal absorption in groups H and M. The serum cortisone and 24 h urine 17-OHCS were significantly reduced in group H. Conclusions Gastric gavage of 10 mL/kg of body weight of a high concentration of a senna leaf decoction (1.0 g/mL) for 4 weeks was used to create a rat model of diarrhea with spleen-kidney yang deficiency syndrome.
Highlights
Diarrhea, characterized as increased stool frequency and loose or watery fecal consistency, is one of the most common gastrointestinal disorders in clinic
Functional diarrhea (FDr) is categorized to the latter according to the Rome IV criteria [2]
The D-xylose absorption test assesses the intestinal capacity to absorb the simple sugar D-xylose as an indicator of adequate nutrient absorption [21] and is commonly used to indicate the condition of spleen yang deficiency [22]. We applied this test with body weight monitoring, food, and water intake measurements to evaluate the success of senna leaf-induced spleen yang deficiency
Summary
Jiajie Zhu ,1,2,3 Shan Liu, Yu Guo ,2 Liwei Hou, Xiaolan Su, Yijie Li, Boyu Han, Dengke Liu, Qingguo Wang ,6 Jiande JD Chen ,7 and Wei Wei 2. Traditional Chinese Medicine Department of Gastroenterology, China-Japan Friendship Hospital, Beijing. College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing. Division of Gastroenterology and Hepatology, Johns Hopkins University, School of Medicine, Baltimore, MD. Received 29 November 2017; Revised 2 March 2018; Accepted 20 March 2018; Published 30 September 2018
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