Abstract
Cottrell chronoamperometry of metabolites relies on a scheme which incorporates dual specificity safeguards of enzymatic catalysis and controlled potential electroylsis. Relevant methodological principles are illustrated by the selective oxidation of β- d-glucose with benzoquinone. Gluconic acid yields of +99.9% were attained in the presence of glucose oxidase. Concomitantly, equivalent amounts of benzoquinone were reduced to hydroquinone, which conveniently was quantitated in quiescent solutions, at stationary palladium thin-film anodes. Sample sizes of 25 μl were used, documenting microclinical applicability.
Published Version
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