Abstract
A new methyl methacrylate embedding medium, Medim K-Plast, is used for histochemical demonstration of acid and alkaline phosphatase activities in undecalcified rat femurs. Fixation, dehydration, and embedding are completed within four days. The method requires no explosive initiator for polymerization, and there are no water bath temperature peaks exceeding 35°C. The method can be adapted to routine laboratory. conditions with a large number of tissue specimens. Short incubation times of 30 to 60 minutes are sufficient to demonstrate enzyme activities by simultaneous coupling azo dye methods. Enzyme activity is retained after storage of blocks for 3 months at 4°C or in sections at −20°C for 4 weeks. Alkaline phosphatase activity for investigation of bone growth is unsatisfactory because the enzyme is associated not only with osteoblasts, but with reticular cells of bony trabeculae. Demonstration of newly formed bone matrix with Solochrome cyanine R staining, therefore, is done without prior deplasticizing of the sections. (The J Histotechnol 10: 103, 1987)
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