Abstract
The success of the newly developed “Specific Methanogenic Activity Test” (SMA-test) in measuring the potential of anaerobic sludge to convert soluble substrate into CH4 and CO2 (James et al., 1990) made this test potentially useful for a variety of investigations. The SMA-test was originally developed for assessment of specific methanogenic activity of anaerobic sludge, but the reliability of the method in detecting even very small changes of sludge activity has drawn attention to its use for many different purposes, especially to evaluate the behaviour of anaerobic sludge when subjected to potentially inhibitory compounds. Recent investigations into the potentially inhibitory effects of the Lithium ion on anaerobic sludge were successfully carried out employing the SMA-test. The test proved to be very sensitive for this type of experiment and the results clearly showed a relationship between the degree of inhibition and the concentration of Lithium (Anderson et al., 1991). This paper describes the methodology for measuring the specific methanogenic activity of anaerobic sludges and discusses how the SMA-test can be used for different purposes.
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