Abstract
To measure the blood volume of the neonate we have developed a new method, which uses liquid chromatographic determination of different hemoglobin subtypes(1). Hemoglobins are separated on a PolyCAT A-cation exchange column (PolyLC Inc.) and detected with a Applied Biosystems absorbance detector. Packed donor red cells (3 ml/kg birth weight) are transfused and samples (0, 1 ml) of infant blood drawn before and 15minutes after transfusion. Hemoglobin F and A0 relative amounts are determined. Based on the change in relative amounts of hemoglobins (as a result of transfusion) and obtained hematocrit values, red blood cell volume and blood volume are calculated.
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